CD14 anticorps

N° du produit ABIN2192076

Cet anticorps anti-CD14 est un anticorps Souris Monoclonal détectant CD14 dans FACS et InhA. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour CD14 anticorps (ABIN2192076)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Inhibition Assay (InhA)

Clone: 18D11

Détail du produit anti-CD14 anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of human sCD14 to be inactivated.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-CD14 (ABIN2192076)

Tunheim, Schjetne, Fredriksen, Sandlie, Bogen: "Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes." dans: Journal of leukocyte biology, Vol. 77, Issue 3, pp. 303-10, (2005) (PubMed).

Wang, Jørgensen, Ellingsen, Almiöf, Thiemermann, Foster, Aasen, Solberg: "Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood." dans: Shock (Augusta, Ga.), Vol. 16, Issue 3, pp. 178-82, (2001) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: The monoclonal antibody 18D11 recognizes human monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells. The monoclonal antibody 18D11 reacts with both soluble and membrane CD14. The monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14, it inhibits LPS induced TNF production. The monoclonal antibody is cross reactive with pig CD14. Together with a C5a neutralizing monoclonal antibody, 18D11 blocks sepsis in a pig model.

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades